Enzyme Technology & Biotransformation Friday, January 14th, 2011

SCHEME 2010
BRANCH BIO-TECHNOLOGY
SUBJECT CODE 10BT-63
SUBJECT NAME Enzyme Technology & Biotransformation

SYLLABUS

PART A
UNIT 1:
INTRODUCTION
Introduction to enzymes, Classification, Sources, Mechanism of enzyme action. Strategies of
purification of enzymes, criteria of purity, molecular weight determination and characterization
of enzymes. 06 Hours
UNIT 2:
BIOCATALYSTS
Advantages of enzymes vs chemical catalysts, Isolated Enzymes versus whole cell systems,
enzymes in fermentation, Biocatalytic Application, Enzyme catalysis (Acid-base, Covalent,
Metal ion catalysis, Substrate strain & entropy effects) Mechanism of coenzymes ( NAD/NADP,
FAD/FADH2, PLP, Coenzyme A, TPP, Biotin) 08 Hours
UNIT 3:
ENZYMES OF BIOLOGICAL IMPORTANCE
Acetylcholinesterase, angiotensin converting enzyme (ACE), ACE Inhibitors, HMG Co A
reductase inhibitors, pseudocholinesterase, 5 -nucleotidase (5NT), glucose-6-phosphate
dehydrogenase (GPD), CKisoforms, immunoreactive trypsinogen (IRT) and chymotrypsin;
amylase isoenzymes, 06 Hours
UNIT 4:
ENZYMATIC TECHNIQUES
Enzyme and isoenzyme measurement methods with two examples (fixed incubation and kinetic
methods); Methods for investigating the kinetics of Enzyme catalysed reactions – Initial velocity
studies, rapid-reaction techniques. Standardization and optimization methods, stability of
enzymes. 06 Hours
PART B
UNIT 5:
IMMOBILIZED ENZYMES
Techniques of enzyme immobilization; kinetics of immobilized enzymes, effect of solute,
partition & diffusion on the kinetics of immobilized enzymes, design and configuration of
immobilized enzyme reactors; applications of immobilized enzyme technology, Economic
argument for immobilization. 08 Hours
38
UNIT 6:
ENZYMATIC TRANSFORMATION
Reaction engineering for enzyme-catalyzed biotransformations. Catalytic antibodies.
Biocatalysts from extreme Thermophilic and Hyperthermophilic microorganisms
(extremozymes). The design and construction of novel enzymes, artificial enzymes,
Biotransformation of drugs (hydroxylation of Steroids), Host Guest Complexation chemistry,
enzyme design using steroid templates. 06 Hours
UNIT 7:
MEDICAL APPLICATIONS
Importance of enzymes in diagnostics, Enzyme pattern in diseases like Myocardial infarctions
(SGOT, SGPT & LDH). Isoenzymes (CK, LD, ALP). Use of isozymes as markers in cancer and
other diseases. Enzymes in immunoassay techniques. Therapeutic enzymes. Inborn errors of
metabolism. 06 Hours
UNIT 8:
INDUSTRIAL APPLICATIONS
Enzymes used in detergents, use of proteases in food, leather and wool industries; methods
involved in production of glucose syrup from starch (using starch hydrolyzing enzymes),
production of maltose and sucrose, glucose from cellulose, uses of lactase in dairy industry,
glucose oxidase and catalase in food industry; Restriction enzymes and DNA ligases. 06 Hours

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