Downstream Process Technology Friday, January 14th, 2011

SCHEME    2006            
BRANCH BIO-TECHNOLOGY
SUBJECT CODE 10BT-73
SUBJECT NAME Downstream Process Technology

SYLLABUS

PART A
UNIT 1. INTRODUCTION
Role and importance of downstream processing in biotechnological processes. Problems and requirements of byproduct purification. Economics of downstream processing in Biotechnology. Cost cutting strategies, Characteristics of biological mixtures, Process design criteria for various classes of byproducts (high volume, low value products and low volume, high value products), Physico-chemical basis of different bio-separation processes.                                 04 Hours
UNIT 2. PRIMARY SEPARATION TECHNIQUES
Cell disruption methods for intracellular products, removal of insolubles, biomass (and particulate debris) separation techniques; flocculation and sedimentation, Centrifugation (ultra and differential) and filtration methods.                                                                        07 Hours
UNIT 3. PRODUCT IDENTIFICATION TECHNIQUES
Principle and Applications of Electrophoresis - their types, Types of staining, Iso-electric focusing, ELISA (Enzyme Linked Immuno Sorbant Assay).                                            05 Hours
UNIT 4. PRODUCT SEPARATION TECHNIQUES - CLASSICAL
Distillation, Liquid - liquid extraction, Absorption and Adsorption, Evaporation.   10 Hours

PART B

UNIT 5. MEMBRANE SEPARATION PROCESSES
Membrane – based separations theory; Design and configuration of membrane separation equipment; Solute polarization and cake formation in membrane ultra filtration – causes, consequences and control techniques; Applications: Use of membrane diffusion as a tool for separating and characterizing naturally occurring polymers; enzyme processing using ultra filtration membranes; separation by solvent membranes; reverse osmosis.                    08 Hours
UNIT 6. ENRICHMENT OPERATIONS
Precipitation methods with salts, organic solvents, and polymers, extractive separations. Aqueous two-phase extraction, supercritical extraction; In situ product removal / integrated bio-processing.                                                                                                                        06 Hours
UNIT 7. PRODUCT RESOLUTION / FRACTIONATION
Adsorptive chromatographic separation processes, Electrophoretic separations, hybrid separation technologies, Dialysis; Crystallization.                                                                       04 Hours
UNIT 8. PRODUCT RECOVERY -  CHROMATOGRAPHIC TECHNIQUES
Partition chromatography - Single dimensional (Both Ascending and Descending) and two dimensional chromatography - Thin layer chromatography, Gas liquid Chromatography, Adsorption Chromatography: Adsorption column chromatography and TLC. Ion Exchange Chromatography: cation Exchange and anion Exchange chromatography. Gel Filtration Chromatography, Hydrophobic interaction chromatography, Affinity Chromatography, High Performance liquid chromatography (HPLC) – analytical and preparative
10 Hours



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